Susceptibility-Matched Multiwell Plates for MRI and NMR Spectroscopy


The technology is a patent estate covering multi-well assay plates for high-throughput screening using MRI and Nuclear Magnetic Resonance (NMR) spectroscopy. Conventional multi-well plates typically give poor performance for MRI-based assays since they provide inadequate matching of magnetic susceptibility between the plate, the sample and their surroundings. This results in distortion of the magnetic field within the scanner and reduces the sensitivity for detecting magnetic particles and the resolution of NMR spectra. The plates of this invention are a one-piece polyetherimide plastic construction for improved magnetic susceptibility matching for aqueous samples. Further, susceptibility matching can be achieved by including plugs, individually or as a cap mat, for each well the same plastic as the plate. The plates, with or without plugs, can be filled and manipulated by standard robotic laboratory equipment widely used in the pharmaceutical industry. Multi-well plates are used in high-throughput research applications such as clinical chemistry, immunology, and drug discovery. MR imaging of multi-well plates in particular offers the possibility of detecting magnetic nanoparticles for use as imaging agents. Moreover, MRI-guided localized NMR spectroscopy could be used to perform detailed chemical analysis of complex mixtures of metabolites not possible by conventional analytical techniques. Best of all, conventional MRI techniques exist which permit all samples in one or more multi-well plate(s) to be analyzed simultaneously.

Potential Commercial Applications: Competitive Advantages:
  • NMR spectroscopy
  • MRI imaging of magnetic nanoparticles
  • Clinical chemistry
  • Immunology
  • Drug discovery, combinatorial chemistry, and quality control in the pharmaceutical, chemical and agricultural industries
 
  • Increased signal-to-noise ratio and sensitivity relative to conventional multi-well plates
  • Portability
  • Compatible with existing high-throughput robots


Inventors:
Kenneth Fishbein (NIA)


Intellectual Property:
US Application No. 60/725,299
U.S. Pat: 8,427,160 issued 2013-04-23


Licensing Contact:
Michael Shmilovich , Esq.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325
Rockville , MD 20852
Email: shmilovm@mail.nih.gov
Phone: 301-435-5019
Fax: 301-402-0220

OTT Reference No: E-243-2005/0

Updated: 03/28/2013